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試劑盒 > 酶聯免疫試劑盒 > 犬皮質醇(Cortisol)ELISA Kit
    • ELISA Kit
    • 犬皮質醇(Cortisol)ELISA Kit
  • 產品類型:
    ELISA Kit
  • 產品名稱:

    犬皮質醇(Cortisol)ELISA Kit

  • 英文名稱:

    Canine Cortisol ELISA Kit

  • 貨號:
    CSB-E14303c
  • 別名:
    N/A
  • 規格:
    96T
  • 中文價格:
    4200
  • 種屬:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 其他種屬:
  • 待測物名稱:
    Cortisol
  • 縮寫:
    COR
  • 蛋白功能1:
    Metabolism
  • 檢測范圍:
    0.049 ng/mL-200 ng/mL
  • 靈敏度:
    0.049 ng/mL
  • 反應時間:
    1-5h
  • 所需樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 下載:
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    200 0.171 0.185 0.178    
    50 0.205 0.209 0.207    
    12.5 0.317 0.293 0.305    
    3.12 0.468 0.482 0.475    
    0.78 0.821 0.878 0.850    
    0.195 0.952 1.006 0.979    
    0.049 1.208 1.200 1.204    
    0 1.609 1.700 1.655    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of canine cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-98  
    1:2 Average % 98  
    Range % 92-106  
    1:4 Average % 83  
    Range % 80-87  
    1:8 Average % 90  
    Range % 83-96  
  • 回收率:
    The recovery of canine cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 87-98  
    EDTA plasma (n=4) 97 92-105  
                 
                 
  • 定性/定量:
    quantitative
  • 研究領域:
    Metabolism