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試劑盒 > 酶聯免疫試劑盒 > 小鼠皮質醇(Cortisol)ELISA Kit
    • ELISA Kit
    • 小鼠皮質醇(Cortisol)ELISA Kit
  • 產品類型:
    ELISA Kit
  • 產品名稱:

    小鼠皮質醇(Cortisol)ELISA Kit

  • 英文名稱:

    Mouse Cortisol ELISA Kit

  • 貨號:
    CSB-E05113m
  • 規格:
    96T
  • 中文價格:
    3800
  • 種屬:
    Mus musculus (Mouse)
  • 其他種屬:
  • 待測物名稱:
    Cortisol
  • 縮寫:
    COR
  • 蛋白功能1:
    Metabolism
  • 檢測范圍:
    0.049 ng/mL-200 ng/mL
  • 靈敏度:
    0.049 ng/mL
  • 反應時間:
    1-5h
  • 所需樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 下載:
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    200 0.177 0.183 0.180    
    50 0.250 0.268 0.259    
    12.5 0.435 0.443 0.439    
    3.12 0.668 0.652 0.660    
    0.78 1.034 1.124 1.079    
    0.195 1.322 1.316 1.319    
    0.049 1.599 1.603 1.601    
    0 2.159 2.012 2.086    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 86-97  
    1:2 Average % 97  
    Range % 92-103  
    1:4 Average % 89  
    Range % 85-92  
    1:8 Average % 90  
    Range % 84-96  
  • 回收率:
    The recovery of mouse cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 88-93  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 定性/定量:
    quantitative
  • 研究領域:
    Metabolism
  • 引用文獻:
    1. Low temperature-aged garlic extract suppresses psychological stress by modulation of stress hormones and oxidative stress response in brain , Hwang KA, et al,JOURNAL OF THE CHINESE MEDICAL ASSOCIATION,2019