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抗體 > 單克隆抗體 > CD19 Monoclonal Antibody
  • 產品類型:
    Monoclonal Antibody
  • 產品名稱:

    CD19 Monoclonal Antibody

  • 貨號:
    CSB-MA004888A0m
  • 規格:
    ¥2,000
  • 種屬:
    Human
  • 免疫原:
    Recombinant Human CD19 protein (20-291AA)
  • 宿主:
    Mouse
  • 種屬反應性:
    Human
  • 應用范圍:
    ELISA, WB, IHC, IF, FC; Recommended dilution: WB:1:2000-1:80000, IHC:1:50-1:200, IF:1:50-1:200
  • 背景:
    Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    IgG1
  • 純化方式:
    >95%, Protein A purified
  • 標記方式:
    Non-conjugated
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式:
    Liquid
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 圖片:


    Western Blot

    Positive WB detected in: Raji whole cell lysate

    All lanes: CD19 antibody at 1:2000

    Secondary

    Goat polyclonal to Mouse IgG at 1/10000 dilution

    Predicted band size: 61 kDa

    Observed band size: 95 kDa



    Western Blot

    Positive WB detected in: Raji whole cell lysate

    All lanes: CD19 antibody at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000

    Secondary

    Goat polyclonal to Mouse IgG at 1/10000 dilution

    Predicted band size: 61 kDa

    Observed band size: 95 kDa



    IHC image of CSB-MA004888A0m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.



    IHC image of CSB-MA004888A0m diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.



    Immunofluorescence staining of Hela cells with CSB-MA004888A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Immunofluorescence staining of Raji cells with CSB-MA004888A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Overlay histogram showing Raji cells stained with CSB-MA004888A0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

  • 說明書:

    注:產品說明書可能有改進,請直接與CUSABIO產品專員聯系,索取最新版說明書!

  • 別名:
    B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19, CD19
  • 指標名稱:
    CD19
  • 克隆號:
    8G11B7