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活性蛋白 > 其他 > Recombinant Human Epidermal growth factor receptor(EGFR),partial (Active)
  • 產品名稱:

    Recombinant Human Epidermal growth factor receptor(EGFR),partial (Active) IN STOCK

  • 貨期:
    3-7 工作日
  • uniprot號:
  • 貨號:
    CSB-MP007479HU
  • 規格:
    ¥1800
  • 圖像:

     



    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.



    Activity

    Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Anti-EGFR recombinant antibody, the EC50 of human EGFR protein is 2.867-3.571 ng/ml.



    Activity

    Human EGF protein captured on COOH chip can bind Human EGFR protein, his and Myc tag (CSB-MP007479HU) with an affinity constant of 11.9pM as detected by LSPR Assay.

  • 測活實驗手冊:
  • 分子結構:
  • 基因名稱:
    EGFR
  • 別名:
    Recommended name:Epidermal growth factor receptor EC=2.7.10.1 Alternative name(s):Proto-oncogene c-ErbB-1 Receptor tyrosine-protein kinase erbB-1
  • 種屬:
    Homo sapiens (Human)
  • 蛋白描述:
    Extracellular Domain
  • 分子量:
    73.6 kDa
  • 標簽信息:
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 來源:
    Mammalian cell
  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 緩沖液:
    Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
  • 內毒素:
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性:
    Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Anti-EGFR recombinant antibody, the EC50 of human EGFR protein is 2.867-3.571 ng/ml.
  • 氨基酸序列:
    LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALN
    TVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQW
    RDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHN
    QCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDH
    GSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPV
    AFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLN
    ITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEG
    CWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAH
    YIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPS
  • 說明書:

    注:產品說明書可能有改進,請直接與CUSABIO產品專員聯系,索取最新版說明書!

  • 蛋白儲存:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
  • 表達區域:
    25-645aa
  • 使用注意:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • 研究領域:
    Cancer